Rat F8 ELISA Kit

Coagulation Factor VIII (FVIII), the glycoprotein encoded by the F8 gene and deficient in hemophilia A, can be quantified in rat specimens with the Assay Genie Rat F8 ELISA Kit, a quantitative sandwich immunoassay built around rat-specific capture and detection antibodies. Targeted at laboratory research in coagulation biology and hemophilia models, the kit provides a colorimetric 96-well microplate workflow with pre-coated wells, recombinant standards, and detection reagents sufficient for 96 individual determinations.

Researchers investigating hemophilia A pathogenesis, FVIII inhibitor development, coagulation factor replacement therapy, and venous thrombosis use the kit to measure circulating FVIII concentrations in rodent samples. Because the capture and detection antibodies are rat-specific, the assay shows no significant cross-reactivity with homologous Factor VIII from human or mouse, supporting preclinical rat studies where background signal would otherwise confound the readout. The microplate format enables batch processing of standards and unknowns together, with TMB-based detection producing an absorbance change proportional to FVIII captured on the well surface.

Each kit includes a coated 96-well plate packaged in a sealed foil pouch and ships with reagents held under refrigeration until first use. The protocol calls for a 50 µL sample volume per well, allowing quantitative readout from small rodent specimens, and the assay is for research use only rather than diagnostic application. Reconstituted standards and working detection antibody should be handled according to the supplied insert to preserve performance across runs.