Rat Extracellular Signal Regulated Kinase 2 (ERK2) ELISA Kit

Researchers investigating the MAPK/ERK signaling cascade in rat models can quantify ERK2 (also called MAPK1 or p42 MAPK) using this colorimetric immunoassay from Assay Genie. The target is a ~42 kDa serine/threonine kinase occupying a central position in the signal transduction cascade that regulates cell proliferation, differentiation, and survival in response to growth factors, cytokines, and stress stimuli.

The rat-specific kit is compatible with tissue homogenates, cell lysates, and cell culture supernatants, enabling measurement of ERK2 expression and pathway activity across diverse experimental contexts. Applications include investigations of neuronal plasticity, oncogenic transformation, cardiac hypertrophy, and inflammatory signaling, where ERK2 phosphorylation status and total protein abundance serve as biomarkers of pathway engagement.

Each kit contains reagents sufficient for a full microplate run. The protocol follows standard ELISA workflows and is adaptable to routine laboratory scheduling. Reagents should be protected from light during handling and use, and the kit should be refrigerated between uses. Researchers are referred to the accompanying product manual for component-level handling details.