Rat Extracellular matrix protein 1 (Ecm1) ELISA Kit

Designed for the quantitative measurement of Extracellular matrix protein 1 (ECM1) in rat samples, this sandwich ELISA kit from Assay Genie accepts serum, plasma, tissue homogenates, cell culture supernates and other biological fluids. It is specific for natural and recombinant rat ECM1, with a stated detection range of 0.312-20ng/mL and a sensitivity of 0.162ng/mL, and it is intended for research use only within the cardiovascular research area.

ECM1 (synonym: Secretory component p85; Uniprot Q62894; NCBI Gene ID 116662; molecular weight 63,249 Da) is a secreted extracellular matrix protein involved in endochondral bone formation as a negative regulator of bone mineralization, which stimulates endothelial cell proliferation and angiogenesis and inhibits MMP9 proteolytic activity. The manufacturer notes that dysregulation of ECM1 has been associated with cancer, fibrosis and autoimmune disorders, supporting its relevance to studies of cell adhesion, migration and tissue development in rat models.

Supplied components include a dismountable microplate, lyophilised standard, sample diluent, assay diluents A and B, detection reagents A and B, wash buffer, substrate, stop solution and plate sealer, with component-specific storage temperatures. Intra-assay CV, inter-assay CV, linearity and recovery data are stated to be provided with the kit.