Rat ET-1 (Endothelin 1) CLIA Kit (RTES00100)

From Assay Genie, this chemiluminescence immunoassay kit is designed for the quantitative measurement of Rat Endothelin-1 (ET-1) in serum, plasma and other biological fluids. The kit uses a sandwich-CLIA format with an assay time of 3 h 30 min, in which an antibody specific to rat ET-1 is pre-coated onto the wells of a microplate, with detection via a biotinylated detection antibody and HRP-conjugated Streptavidin. Relative Light Units (RLU) are read on a chemiluminescence immunoassay analyzer, with values directly proportional to the concentration of the target analyte and determined by reference to a standard curve.

Key analytical specifications include a detection range of 3.13-200 pg/mL, a sensitivity of 1.88 pg/mL, and a stated recovery range of 80%-120%. The recommended sample volume is 100 μL. The manufacturer reports no significant cross-reactivity or interference between rat ET-1 and analogues, and the associated UniProt ID is P22388. Reported intra-assay precision CV values range from 10.33% to 11.32% and inter-assay CV values from 9.02% to 12.85%. The assay is intended for research use only and is relevant to studies in cancer, cardiovascular biology, metabolism, and signal transduction. Components are stored at 2-8°C or -20°C as indicated, and the kit is shipped on ice packs.

Additional materials required (not supplied) include a 37°C incubator, a plate reader, precision pipettes with disposable tips, distilled water, disposable tubes for sample dilution, and absorbent paper. The kit also includes a Bradford Reagent for total protein quantification to support sample normalization.