Rat ER beta/ESR2/Estrogen Receptor beta ELISA Kit

Designed by Assay Genie, this Sandwich ELISA kit with double-antibody detection provides quantitative measurement of estrogen receptor beta (ESR2) in rat samples. The colorimetric assay is validated for serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.

Reported linearity for serum at 1:2, 1:4, and 1:8 dilutions is 90-105%, 82-94%, and 80-95% respectively; EDTA plasma shows 91-103%, 82-97%, and 81-99%; heparin plasma shows 93-99%, 83-95%, and 80-99%. Recovery averages 93% (serum), 95% (EDTA plasma), and 96% (heparin plasma). The research area is listed as Epigenetics and Nuclear Signaling, and the target is associated with UniProt accession Q62986.

The protocol involves equilibrating reagents to room temperature, a 90-minute primary incubation at 37°C, a 60-minute biotin-labeled detection antibody step, a 30-minute HRP-Streptavidin binding step, TMB color development in the dark for 10-20 minutes, and absorbance reading at 450 nm. Reagents should be stored at 2-8°C for 12 months, with light-sensitive components protected from direct light.