Rat Enolase, Non Neuronal (NNE) ELISA Kit

Quantitative immunoassay from Assay Genie for measuring non-neuronal enolase (alpha-enolase, ENO1) in rat specimens using a microplate workflow. The kit targets the glycolytic enzyme that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate, distinct from neuron-specific enolase (NSE/ENO2), and supports research into cancer biology, inflammatory disease, and metabolic disorders where ENO1 surface expression and plasminogen binding have been implicated.

Each kit provides reagents for 96 assays and is configured for colorimetric readout on a standard plate reader. The assay is validated for use with rat-derived serum samples and plasma specimens, with protocol steps suitable for routine laboratory workflows. ENO1 is studied as a candidate biomarker and autoantigen in rat models of lung injury, rheumatoid arthritis, and tumorigenesis, making this ELISA a useful tool for translational research programs that require species-matched quantitative data.

Intended for research use only, the kit should be stored refrigerated and brought to room temperature before use. Researchers should consult the supplied protocol for preparation of standards, sample dilution guidelines, and recommended dilution buffers, as well as for the specific working concentrations of detection antibody and streptavidin-HRP conjugate provided with the kit.