Rat Elastase 2, Neutrophil (ELA2) ELISA Kit

Neutrophil Elastase, the serine protease encoded by ELANE and commonly designated Elastase 2 or ELA2, is stored in the azurophilic granules of circulating neutrophils where it cleaves elastin and other extracellular matrix substrates during pathogen clearance and tissue remodelling. The Assay Genie rat ELA2 immunoassay delivers a quantitative, absorbance-based read-out for investigators profiling neutrophil degranulation and neutrophil extracellular trap (NET) formation in rat models of acute lung injury, sepsis, chronic obstructive pulmonary disease, emphysema, atherosclerosis, inflammatory bowel disease, and wound healing. Supplied as a 96-assay microplate with pre-coated strip wells, the kit accepts serum preparations, plasma specimens, and tissue homogenate samples drawn from rat cohorts, supporting both longitudinal biomarker studies and ex vivo stimulation experiments. Each pack contains the coated plate, biotinylated detection antibody, recombinant protein standard, dilution buffer, wash concentrate, chromogenic substrate, and stop reagent sufficient for 96 single-use determinations read on a standard plate reader. Researchers frequently pair the assay with elastase activity probes and myeloperoxidase measurements to characterise protease-antiprotease imbalance in translational pharmacology and respiratory disease research, and it is intended for research use only.