Rat EGF (Epidermal Growth Factor) CLIA Kit (RTES00202)

The Rat EGF (Epidermal Growth Factor) CLIA Kit from Assay Genie uses a sandwich chemiluminescence immunoassay to quantify endogenous EGF concentrations in serum, plasma, and other biological fluids. Epidermal Growth Factor is a 53 amino acid peptide that binds the EGFR receptor and drives epithelial cell proliferation, tissue repair, gastric mucosal protection, and salivary gland secretion, making it a frequent analyte in wound healing, gastrointestinal, and nephrology research.

The kit is supplied as a 96-well microplate with pre-coated capture antibodies, lyophilized standards, biotinylated detection antibody, and chemiluminescent substrate working solution. Researchers typically load 50 µl of sample per well, with assay sensitivity in the low pg/ml range and a standard curve covering a broad dynamic range. Chemiluminescent readout delivers a higher signal-to-noise ratio than colorimetric ELISA, supporting low-abundance detection in small-volume rodent samples.

Store all components at 2-8 °C, protect the substrate from light, and allow reagents to equilibrate to room temperature before use. For long-term storage, keep lyophilized standards frozen at -20 °C and limit freeze-thaw cycles by aliquoting. Intended for research use only; not for diagnostic or clinical applications.