Rat E2 (Estradiol) ELISA Kit (RTFI01469)

This competitive ELISA kit from Assay Genie provides quantitative in vitro measurement of estradiol (E2) in laboratory rat specimens. Using a competitive immunoassay architecture, the assay pairs an estradiol-specific antibody with an enzyme-labeled conjugate to produce a measurable signal that is read on a standard microplate reader.

Estradiol is a principal estrogen steroid hormone, and rodent models in endocrinology, reproductive biology, and toxicology rely on accurate hormone quantification in experimental rat samples. The reagent set supports research into estrous cycle regulation, ovariectomy and gonadectomy models, hormone replacement protocols, and pharmacological studies of the hypothalamic-pituitary-gonadal axis.

The kit supplies all reagents needed for a full microplate run. Components should be brought to room temperature before starting the protocol, and any unused strip wells resealed with desiccant to preserve reagent stability. The competitive format produces an inverse standard curve, where the measured signal decreases as estradiol concentration rises across the calibrated range.