Rat Drebrin 1 (DBN1) ELISA Kit

Quantitative measurement of Drebrin 1 (DBN1) in rat specimens is the focus of this Assay Genie ELISA kit, which uses a paired-antibody capture format for detection of the neuronal actin-binding protein. DBN1 is the neuron-enriched isoform that supports synaptic cytoskeleton organization and is distinct from the broadly expressed DBN2 form. Researchers studying neuronal plasticity, cytoskeletal remodeling, or neurodegenerative disease models apply the assay to profile endogenous DBN1 levels across tissue homogenates and other experimental rat samples.

Supplied as a 96-assay microplate kit with absorbance-based readout, the workflow relies on paired antibodies that recognize distinct epitopes of rat DBN1, supporting quantification against a calibrated standard curve. The microplate format fits standard plate readers used in immunology and neuroscience laboratories. Each kit ships with the pre-coated plate, biotinylated detection antibody, streptavidin conjugate, standards, wash buffer, and substrate required to complete the full assay workflow from start to finish.

Standard immunoassay practice applies: equilibrate reagents to room temperature before use, run standards and samples in duplicate, and read absorbance within the linear portion of the standard curve. Storage: keep kit components refrigerated until first use and protect from prolonged light exposure to preserve activity through repeated freeze-thaw cycles if standards are aliquoted into smaller volumes.