Rat Dkk-2/Dickkopf-related protein 2 ELISA Kit

Assay Genie's Rat Dkk-2/Dickkopf-related protein 2 ELISA Kit provides a sandwich, double-antibody configuration for the quantitative measurement of DKK-2 in rat samples. Using colorimetric detection, the assay supports serum, plasma, cell culture supernatant, cell or tissue lysates, and other liquid samples, with a reported sensitivity of 0.188 ng/mL and a working range of 0.313-20 ng/mL. Unopened kits are stored at 2-8°C for 12 months.

DKK-2 is a member of the Dickkopf family of proteins that regulate the Wnt signaling pathway and influence cell differentiation and proliferation. Dysregulation of DKK-2 has been implicated in cancer, osteoporosis, and Alzheimer's disease, highlighting its relevance as a research biomarker and therapeutic target. The protocol pairs a biotin-labelled detection antibody with HRP-Streptavidin (SABC) and TMB substrate, with absorbance measured at 450 nm.

Validation data cover linearity at 1:2, 1:4, and 1:8 dilutions for serum, EDTA plasma, and heparin plasma samples, alongside recovery ranges for each matrix. The kit is suited to researchers investigating DKK-2 biology, Wnt pathway function, and related disease models.