Rat Deiodinase, Iodothyronine, Type I (DIO1) ELISA Kit

For quantitative measurement of type I iodothyronine deiodinase (DIO1) levels in rat-derived specimens, this colorimetric immunoassay from Assay Genie provides a microplate-based workflow with all required reagents pre-measured for a complete run. Researchers studying peripheral thyroid hormone metabolism apply this kit to tissue homogenate preparations, supporting investigations into hepatic and renal T4-to-T3 conversion, selenoenzyme regulation, and endocrine disruption in rodent models.

The procedure follows an indirect-detection protocol with absorbance readings captured on a compatible plate reader at the recommended wavelength. Each kit supplies pre-coated wells, assay diluent, wash buffer, calibrated standard, detection antibody, and substrate solution sufficient for ninety-six determinations run in parallel.

Storage recommendations apply to unopened components; refrigerated reagents should equilibrate to room temperature before initiating the protocol. Reagent stability supports reproducible results across a typical experimental timeline, and the shelf life is suitable for routine laboratory scheduling. Validated specifically for rat samples, the assay is intended for research use only and is supplied in a standard kit configuration.