Rat DDAH2 ELISA Kit

Assay Genie offers a Sandwich ELISA kit designed for the quantitative measurement of DDAH2 (Dimethylarginine Dimethylaminohydrolase 2) in rat samples. The kit uses a colorimetric detection method based on a double-antibody sandwich format, with absorbance read at 450 nm. DDAH2 is an enzyme that plays a key role in regulating nitric oxide production, supporting research into cardiovascular health, hypertension, diabetes, and kidney disorders in rat models.

Validated for use with serum, plasma, cell culture supernatant, cell or tissue lysates, and other liquid samples, the kit is described by the manufacturer as offering high sensitivity and specificity for dependable, reproducible results. Kit components should be stored at 2-8°C for up to 12 months, with certain items storable at -20°C for longer periods.

Performance figures reported by the manufacturer include a sensitivity of 0.188 ng/mL and an assay range of 0.313-20 ng/mL. Linearity has been validated in serum (87-99% at 1:2, 82-101% at 1:4, 85-105% at 1:8, n=5), EDTA plasma (85-105%, 87-96%, 88-104%, n=5) and heparin plasma (81-91%, 84-92%, 84-98%, n=5). Recovery ranges are 89-105% for serum (average 99%), 89-104% for EDTA plasma (average 98%), and 85-104% for heparin plasma (average 96%). The associated UniProt entry is Q6MG60, and reported synonyms include DDAH, DDAHII, G6a, NG30, S-phase protein, Protein G6a, and Dimethylargininase-2.