Rat Cytokine Receptor Like Factor 1 (CRLF1) ELISA Kit

CRLF1 is a secreted cytokine receptor subunit that pairs with cardiotrophin-like cytokine factor 1 (CLCF1) to engage the ciliary neurotrophic factor receptor complex, activating JAK/STAT and MAPK signaling cascades in responsive cells. The Assay Genie rat CRLF1 ELISA Kit provides a microplate-based immunoassay for quantifying this receptor protein in rat-derived laboratory samples, supporting investigation of motor neuron survival, neuromuscular junction formation, and congenital disorders such as cold-induced sweating syndrome type 1, which arises from CRLF1 loss-of-function mutations.

Typical applications include measuring CRLF1 concentrations in tissue homogenates and cell culture supernatants from rodent disease models, as well as profiling receptor expression under neuroinflammatory and immune regulatory conditions. Each kit is configured for a 96-reaction microplate layout with reagents for standard plate-reader workflows, where absorbance values are recorded at the assay-appropriate wavelength.

Unopened components should be stored refrigerated per the product insert, with reconstituted standards and detection antibodies kept on ice during the procedure and protected from light where indicated. Reconstituted reagents are recommended for use within the working day and are not intended for repeated freeze-thaw cycles. The kit is validated specifically for rat samples and is intended for research use only, not for clinical or diagnostic procedures.