Rat Cytochrome P450 2C19 (CYP2C19) ELISA Kit

This quantitative ELISA kit from Assay Genie enables measurement of cytochrome P450 2C19 (CYP2C19) protein levels in rat-derived research samples. CYP2C19 is a phase I hepatic monooxygenase within the cytochrome P450 superfamily that oxidizes a broad spectrum of clinically relevant xenobiotics, including proton pump inhibitors, tricyclic antidepressants, certain benzodiazepines, and the antiplatelet prodrug clopidogrel. Reliable quantitation of CYP2C19 in preclinical rat models supports translational pharmacology research, drug-drug interaction screening, hepatic enzyme induction studies, and metabolic phenotyping experiments.

The reagent set ships with a pre-coated microplate, biotinylated detection antibody, streptavidin-enzyme conjugate, chromogenic substrate, stop solution, concentrated wash buffer, and a calibrated recombinant rat CYP2C19 standard for constructing the standard curve. The kit is configured for use with tissue homogenates, cell lysates, and cell culture supernatants. Researchers should reconstitute the standard according to the supplied protocol and store reconstituted aliquots frozen to minimize freeze-thaw cycles. Unopened components are shipped on cold packs and held refrigerated until first use.

The assay follows a standard immunometric ELISA workflow with absorbance measurement. Intended for research use only; not validated for diagnostic, therapeutic, or veterinary applications.