Rat Cytochrome P450 1A1/CYP3A2 ELISA Kit

Assay Genie's Sandwich ELISA Kit is designed for the accurate measurement of Cytochrome P450 1A1 in rat samples, supporting studies in drug metabolism, toxicology, and pharmacokinetics. Cytochrome P450 1A1 is an important enzyme involved in the metabolism of foreign compounds, including drugs, toxins, and carcinogens, and it plays a key role in drug metabolism and bioactivation, making it a valuable biomarker for studying drug interactions and toxicity in preclinical research.

The kit uses a colorimetric detection method in a double antibody sandwich ELISA format, and is compatible with serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. It provides high sensitivity and specificity, allowing for reliable and reproducible results across a range of research applications.

Performance characteristics include a sensitivity of 0.375 ng/mL and an assay range of 0.625-40 ng/mL. Linearity has been validated in serum (95-105% at 1:2, 82-94% at 1:4, 84-100% at 1:8), EDTA plasma (86-104%, 85-100%, 90-99%), and heparin plasma (86-100%, 83-100%, 81-100%). Recovery values average 99% for serum, 95% for EDTA plasma, and 95% for heparin plasma. The kit should be stored at 2-8°C for 12 months.