Rat Cystatin-B (Cstb) ELISA Kit

Manufactured by Assay Genie, this colorimetric sandwich ELISA kit is designed for the quantitative measurement of rat cystatin-B (CSTB) in rat-derived samples, including serum, plasma, tissue homogenates, cell culture supernates and other biological fluids. The assay detects natural and recombinant rat cystatin-B with a sensitivity of 0.064 ng/mL and a detection range of 0.156–10 ng/mL.

Manufacturer-reported performance figures include an intra-assay CV of 4.9% and an inter-assay CV of 6.8%. Linearity has been assessed in serum, EDTA plasma and heparin plasma across dilutions from 1:2 to 1:16, while recovery in serum averages 90% (84–96%) and in plasma averages 92% (86–98%). According to the product overview, cystatin B plays a role in regulating cysteine protease activity, and its dysregulation has been linked to cancer, neurodegenerative disorders and inflammatory conditions.

Cystatin-B is described as an intracellular thiol proteinase inhibitor (UniProt P01041) that inhibits cathepsins B, H, L and S and may protect against apoptosis in response to seizures. The protein is also known as Cystatin-beta, Liver thiol proteinase inhibitor, Stefin-B and Cst6, is localised to the cytoplasm, has a molecular weight of 11,196 Da and belongs to the cystatin family. The kit is supplied for research use within the epigenetics research area.