Rat CYP21A1 (Steroid 21-hydroxylase) ELISA Kit (RTFI01460)

CYP21A1 encodes steroid 21-hydroxylase, a cytochrome P450 enzyme essential for adrenal biosynthesis of cortisol and aldosterone. Dysregulation of this enzyme is implicated in congenital adrenal hyperplasia, autoimmune adrenal insufficiency, and altered glucocorticoid metabolism in rodent models.

This quantitative immunoassay from Assay Genie enables researchers to measure CYP21A1 protein levels in rat specimens, supporting studies in endocrinology, adrenal physiology, and steroidogenesis pathway analysis. The kit is supplied as a complete 96 Assays reagent set for in vitro research use only, with all buffers, plates, and standards prepared for direct application in standard laboratory workflow.

Each kit supports measurement across the dynamic range needed for tissue lysate and serum-based investigations. The procedure uses a standard microplate reader, and results are typically analyzed within a single working day. Storage at 4°C is recommended for unopened components, with separate handling guidance for reconstituted reagents.