Rat CYP1B1 (Cytochrome P450, family 1, subfamily B, polypeptide 1) ELISA Kit (RTES01088)

CYP1B1 is an extrahepatic cytochrome P450 enzyme that catalyzes the hydroxylation of 17β-estradiol and the bioactivation of polycyclic aromatic hydrocarbon procarcinogens. In rat models, CYP1B1 expression dominates in steroidogenic tissues such as the adrenal cortex, ovaries, and testes, making it a target in reproductive toxicology and hormone-driven cancer research. The Assay Genie kit provides a quantitative colorimetric readout for monitoring CYP1B1 protein abundance across treated and control cohorts, with applications spanning hepatic and extrahepatic metabolism studies, drug-induced enzyme regulation, and ocular hypertension models linked to CYP1B1 loss-of-function mutations.

Each kit contains a precoated microplate, biotinylated detection antibody, and calibrated standards sufficient for up to 96 determinations. Researchers commonly pair this assay with qPCR or western blot validation to correlate CYP1B1 protein levels with transcript and enzyme activity data. Store unopened kits refrigerated and avoid repeated freeze-thaw cycles of reconstituted reagents.