Rat CYP1A1 (Cytochrome P450, family 1, subfamily A, polypeptide 1) ELISA Kit (RTES01086)

The CYP1A1 enzyme, encoded by the cytochrome P450 family 1 subfamily A polypeptide 1 gene, mediates phase I biotransformation of polycyclic aromatic hydrocarbons, heterocyclic amines, and several pharmaceutical substrates. Researchers measure CYP1A1 levels to track hepatic enzyme induction, evaluate responses to dioxin-like compounds, and probe environmental pollutant exposure in rat toxicology models.

This kit from Assay Genie provides 96 assays per plate and is supplied for research use only. It is applied to rat-derived samples to study chemically induced changes in hepatic drug-metabolizing capacity, including investigations of beta-naphthoflavone-, 3-methylcholanthrene-, and aryl hydrocarbon receptor ligand-driven induction pathways.

Each pack contains a pre-coated microplate, capture and detection antibodies, lyophilized standards, substrate solution, wash buffer, and stop reagent. Plates are read on a standard microplate reader; unopened kits are refrigerated and protected from light to preserve activity through the stated shelf life. Reconstituted standards and detection antibody should be aliquoted to limit repeated freeze-thaw cycles and stored refrigerated between runs.