Rat CYP17A1 ELISA Kit

Manufactured by Assay Genie, this sandwich ELISA kit employs a double-antibody format with colorimetric detection to quantify rat cytochrome P450 17A1 (Cyp17a1) in serum, plasma, and tissue homogenate samples. It is intended for research use only and supports studies of steroidogenesis and hormone regulation.

Performance characteristics reported in the specification include a sensitivity of 0.375 ng/ml and a working range of 0.625–40 ng/ml. Spike-and-recovery testing yielded average recoveries of 96% in serum (range 89–102%), 99% in EDTA plasma (range 96–104%), and 96% in UFH plasma (range 90–102%). Linearity testing at 1:2, 1:4, and 1:8 dilutions produced ranges of 85–101%, 90–100%, and 88–103% in serum; 85–101%, 86–95%, and 84–101% in EDTA plasma; and 84–95%, 80–100%, and 83–99% in UFH plasma. Intra-assay precision is stated as CV <8% and inter-assay precision as CV <10%.

The kit is supplied for storage at 4°C, where the manufacturer indicates it remains stable for up to six months. Associated reference identifiers include UniProt entry P11715, NCBI Gene ID 25146 (accession NP_036885.1; reported molecular weight 57,250 Da), and KEGG entry K00512.