Rat CYP11B1 (Cytochrome P450 11B1, mitochondrial) ELISA Kit (RTFI01496)

Developed by Assay Genie, this quantitative immunoassay enables precise measurement of CYP11B1, the mitochondrial steroidogenic enzyme that catalyzes the 11β-hydroxylation of 11-deoxycorticosterone to corticosterone in the rat adrenal cortex. The kit supports research into glucocorticoid biosynthesis, adrenal cortical function, the hypothalamic-pituitary-adrenal (HPA) axis, and experimental models of endocrine hypertension. Pre-coated microplate wells, matched antibody pairs, and serial standard diluents are supplied for a complete plate run.

Reconstituted standards and prepared samples should be aliquoted to limit freeze-thaw cycles, and unused components are stored refrigerated in the dark to preserve reagent activity. The procedure uses 50 µL of serum or plasma per well, with overnight incubation at 4°C and a chromogenic read at the recommended wavelength. Rat-specific capture and detection antibodies support accurate quantification of CYP11B1 without significant cross-reactivity to homologous rat steroidogenic enzymes.