Rat Cyclin Dependent Kinase Inhibitor 1A (CDKN1A) ELISA Kit

CDKN1A, also known as p21, is a key cell-cycle brake protein whose expression in rat models is widely tracked in oncology, toxicology, and aging research. This ELISA kit from Assay Genie is calibrated for rat specimens and accepts tissue homogenates, cell lysates, and clarified supernatant fractions, giving laboratories flexibility across diverse sample preparation workflows. The kit of 96 assays is configured in a standard microplate format suited to moderate-throughput studies comparing control and treatment cohorts, with absorbance read on a conventional plate reader. Each package supplies the pre-coated plate, lyophilized standards, biotinylated detection antibody, streptavidin-HRP conjugate, wash buffer, substrate solution, and stop reagent required to complete the protocol from sample loading through final quantification. Researchers frequently pair CDKN1A readouts with cell proliferation markers such as Ki-67 and PCNA, alongside apoptosis indicators like cleaved caspase-3, to distinguish cell-cycle arrest from programmed cell death following exposure to candidate drugs, DNA-damaging agents, or oxidative stressors. The reagents are intended for research use only and are not validated for diagnostic procedures.