Rat Cyclin D3/CCND3 ELISA Kit

Assay Genie offers a Sandwich (Double Antibody) ELISA kit for the quantitative measurement of Cyclin D3 (CCND3) in rat samples. The assay is validated for serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, with a sensitivity of 18.75 pg/mL and an assay range of 31.25-2000 pg/mL.

The workflow uses a pre-coated microplate in which samples and standards are incubated, followed by a biotinylated detection antibody and HRP-conjugated Streptavidin. Color is developed using TMB substrate and read at 450 nm. Components are stored at 2-8°C for 12 months and the kit includes a Bradford Reagent for total protein normalization. The assay is intended for research use only.

Reported linearity across 1:2, 1:4 and 1:8 dilutions ranges from 82-104% for serum, EDTA plasma and heparin plasma (n=5). Recovery across the same sample types averages between 90% and 96%.