Rat CRH (Corticotropin Releasing Hormone) ELISA Kit

Developed by Assay Genie, this competitive ELISA kit is designed to quantify Corticotropin-Releasing Hormone (CRH) in rat-derived samples with high precision and reproducibility, supporting research in stress-related disorders and neuroendocrinology where CRH plays a central role in regulating the body's stress response.

The assay uses a competitive, antigen-coated format with colorimetric readout at 450 nm. It reports a sensitivity of 0.188 ng/mL and a measuring range of 0.313-20 ng/mL, and is validated for serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The kit is stored sealed at 2-8°C and should not be cryopreserved.

Validated linearity at 1:2, 1:4 and 1:8 dilutions falls between 80% and 105% across serum, EDTA plasma and heparin plasma matrices. Spike-recovery ranges are 91-96% for serum (average 93%), 89-105% for EDTA plasma (average 98%), and 86-97% for heparin plasma (average 93%).