Rat CRF2R/Corticotropin Releasing Factor Receptor 2 ELISA Kit

Manufactured by Assay Genie, this colorimetric sandwich ELISA is designed for the quantitative detection of rat Corticotropin-Releasing Factor Receptor 2 (CRF2R). The double-antibody sandwich format is validated for serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The assay reports a sensitivity of 0.188 ng/mL and a working range of 0.313-20 ng/mL. Reagents are stored at 2-8°C, with lyophilized standards and the microplate stable for 12 months at -20°C.

CRF2R (synonyms: CRH-R-2, Crhr2) plays a critical role in mediating the physiological stress response and is involved in various behavioural and neuroendocrine functions. Dysregulation of CRF2R has been linked to stress-related disorders such as anxiety and depression, supporting the value of accurate quantification in rat models. The supplied protocol proceeds through reagent equilibration, primary incubation at 37°C for 90 minutes, biotinylated detection antibody binding, HRP-streptavidin (SABC) binding, TMB colour development in the dark, and absorbance measurement at 450 nm.