Rat Cortisol PharmaGenie ELISA Kit (SBRS0126)

Endogenous cortisol levels in rat specimens can be quantified with the Rat Cortisol PharmaGenie ELISA Kit from Assay Genie, a competitive immunoassay kit that uses absorbance-based end-point readout on a standard plate reader. Each kit contains the full reagent set needed for a single microplate run, including pre-coated wells, calibrator standards, enzyme conjugate, substrate solution, wash buffer, and stop reagent.

The assay accommodates cell culture supernatant from rat adrenal cell models and specimens collected from rat subjects in vivo, supporting applications across adrenal cell biology studies, ex vivo perfusion experiments, and live-animal sampling from rodent models of stress, inflammation, and metabolic disease. Because the format is competitive, signal decreases as cortisol concentration rises, with quantitation derived from a standard curve generated from the kit's calibrators run in parallel.

Cortisol plays a distinct role in rat endocrinology, where it serves as the dominant circulating glucocorticoid rather than corticosterone, making this kit a targeted tool for translational research on HPA-axis regulation, circadian rhythm, and adrenal function. Each kit ships in insulated packaging with cold packs to maintain reagent integrity during transit, and individual vials of standards and conjugate should be stored refrigerated between uses according to the package insert. The protocol uses small sample aliquots per well, and the competitive design reduces matrix interference relative to direct-binding formats commonly used for low-molecular-weight steroid hormones.

This product is supplied as a single-use research kit and is intended for research use only, not for diagnostic or clinical applications.