Rat Corticotropin Releasing Hormone Receptor 1 (CRHR1) ELISA Kit

Quantitative detection of the CRHR1 receptor in rat-derived samples supports research into the hypothalamic-pituitary-adrenal (HPA) axis and the neurobiology of stress. Corticotropin-releasing hormone receptor 1 is a G protein-coupled receptor that mediates CRF signaling in the brain and periphery, making it a relevant target in studies of anxiety, depression, and endocrine regulation. This Assay Genie kit is validated for rat tissue homogenates and cell lysates, enabling researchers to quantify receptor levels in hypothalamus, pituitary, adrenal, and other tissues of interest. The 96-assay format provides sufficient throughput for mid-sized studies, and the assay follows a standard ELISA workflow intended for in vitro research use only. Samples are typically prepared by homogenizing tissue in an appropriate lysis buffer, clarifying by centrifugation, and loading the clarified lysate directly into the microplate wells. Detection relies on an antibody pair raised against rat CRHR1, and the kit is intended for laboratory research applications such as receptor expression profiling, ligand-binding follow-up work, and pharmacological screening of modulators along the CRF signaling pathway.