Rat Corticotropin Releasing Hormone (CRH) ELISA Kit

The competitive ELISA format used by this Assay Genie kit is well-suited for quantifying the 41-amino acid Corticotropin Releasing Hormone (CRH) peptide, the principal hypothalamic driver of the hypothalamic-pituitary-adrenal (HPA) axis and a central mediator of the mammalian stress response. Researchers apply this rat CRH ELISA to neuroendocrine studies of HPA axis regulation, behavioral correlates of anxiety and depression, pharmacological investigations of CRH receptor antagonists, and experimental models of early-life adversity, inflammation, and metabolic disease. Because CRH circulates at low picomolar concentrations and is rapidly degraded by plasma peptidases, sample handling is critical: collect blood into chilled tubes containing aprotinin or other protease inhibitors, separate serum or plasma promptly by cold centrifugation, aliquot to prevent repeated freeze-thaw cycles, and store at -80 °C until the assay is performed. The kit provides reagents for 96 determinations in a microplate layout and is intended for research use only in laboratory investigations of rodent stress biology, neuropharmacology, and reproductive or metabolic endocrinology.