Rat Coronin 1A (CORO1A) ELISA Kit

Researchers studying actin cytoskeleton dynamics in immune cells rely on quantitative protein measurement to complement gene expression and imaging data. The Assay Genie Rat Coronin 1A (CORO1A) ELISA Kit enables quantitative measurement of CORO1A protein in rat tissue homogenates and cell lysates, supporting studies where antibody-based detection of this actin-binding protein is needed across many samples.

Coronin 1A is a member of the conserved coronin family of actin-binding proteins and shows particularly strong expression in hematopoietic lineages including T lymphocytes, B lymphocytes, neutrophils, and macrophages. The protein regulates actin filament remodeling, cell migration, phagocytosis, and immune synapse formation, and loss-of-function variants in humans are associated with severe combined immunodeficiency. Related research applications include T-cell signaling studies, microglial activation in neuroinflammation models, host-pathogen interaction work, and characterization of leukocyte motility in autoimmune disease contexts.

The kit is supplied as a 96-assay microplate format with reagents packaged in individual vials, providing a standard curve and detection antibodies for parallel processing of unknown samples. It is intended for research use only and is validated for rat samples. Typical workflows involve homogenizing tissue in cold lysis buffer, clarifying by centrifugation, diluting samples, and loading them onto precoated plate wells alongside serial dilutions of the provided standard. The kit supports quantitative comparisons across treatment groups, knockout versus wild-type comparisons, and time-course experiments in laboratories focused on cytoskeletal regulation, immune cell development, or comparative genotypes.