Rat Connexin 40/GJA5 ELISA Kit

A sandwich ELISA kit from Assay Genie designed for the quantitative measurement of Connexin 40 (GJA5) in rat samples. The double-antibody assay uses a colorimetric readout read at 450 nm and is validated for serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.

The kit reports a sensitivity of 23.438 pg/mL and a working range of 39.063–2500 pg/mL, with the target protein indexed as Uniprot P28234. Reagents are shipped on ice packs and stored at 2–8°C for 12 months. The workflow follows a standard sandwich ELISA in which capture antibody is pre-coated onto the wells, a biotin-conjugated detection antibody is added, HRP-Streptavidin is introduced, and TMB substrate development is terminated with stop solution before absorbance is read.

Connexin-40 is described as a key protein involved in cell-to-cell communication and is particularly important in cardiac tissue, where it plays a role in the coordination of heartbeats, making this kit of interest for researchers studying cardiovascular disease, arrhythmias, and other conditions related to Connexin-40 dysfunction.