Rat Complement factor D/Factor D ELISA Kit

Designed for the quantitative measurement of complement factor D in rat samples, this colorimetric sandwich ELISA from Assay Genie uses a double-antibody format and accepts serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid sample types.

Validated performance figures include an assay sensitivity of 0.375 ng/mL and a working range of 0.625–40 ng/mL. In spiking studies using serum (n = 5), EDTA plasma (n = 5) and heparin plasma (n = 5), average recoveries ranged from 97% to 100%, with recovery ranges of 86–104%, 95–104% and 95–103% respectively. Kit reagents should be stored at 2–8°C for 12 months.

Complement factor D is described as a key enzyme of the alternative complement pathway that acts by cleaving C3 into its active form during the immune response. The kit supports research into the role of complement factor D dysregulation in inflammatory and autoimmune disease.