Rat Collagen alpha-1 (XXVII)chain/COL27A1 ELISA Kit

A sandwich ELISA, double antibody kit from Assay Genie designed for the precise measurement of collagen alpha-1(XXVII) chain (COL27A1) levels in rat samples, including serum, plasma, cell culture supernatants, cell or tissue lysates, and other liquid samples. The assay uses a colorimetric detection method, providing accurate and dependable results for a variety of research applications.

The kit offers a sensitivity of 0.938 ng/mL and a detection range of 1.563-100 ng/mL. Reported linearity (1:2 / 1:4 / 1:8 dilutions) spans 86-101% / 83-101% / 81-100% for serum, 90-105% / 84-101% / 80-96% for EDTA plasma, and 89-100% / 82-100% / 90-98% for heparin plasma. Spike-and-recovery performance averages 97% for serum, 99% for EDTA plasma, and 96% for heparin plasma.

All reagents should be stored at 2-8°C for up to 12 months. Collagen alpha-1(XXVII) is a component of the extracellular matrix involved in tissue development, maintenance, and repair, with dysregulation implicated in musculoskeletal disorders, connective tissue diseases, and fibrotic disorders, making this kit a valuable resource for rat model research into collagen biology.