Rat COL4 (Collagen Type ?) ELISA Kit (RTFI01478)

Quantitative measurement of Collagen Type IV in rat-derived samples supports research into basement membrane biology, diabetic nephropathy, and renal fibrosis. Assay Genie's Rat Collagen Type IV ELISA kit enables this application, providing a plate-based immunoassay configured for COL4 detection in rat studies.

Collagen Type IV forms the principal structural scaffold of basement membranes, and altered COL4 deposition is a hallmark of glomerular basement membrane thickening in diabetic nephropathy, progression of renal fibrosis, and remodeling of the extracellular matrix in vascular injury models. Investigators using rat tissues, including kidney cortex, lung, and vascular preparations, employ this analyte to monitor disease progression and treatment response in preclinical workflows.

The plate-based immunoassay is supplied with all reagents needed for the full run, including a pre-coated microplate, biotinylated detection reagent, streptavidin-HRP conjugate, wash buffer, substrate solution, and stop reagent prepared in calibrated volumes. Researchers add standards and unknown samples to designated wells, follow the included protocol, and read absorbance to interpolate COL4 concentrations against the standard curve.

Reagents should be equilibrated to ambient temperature before use, and kit components kept refrigerated in the dark when not in active use to preserve stability across the shelf life of the product. Each kit supports a defined number of determinations per plate, and the format accommodates duplicate or triplicate measurement of unknown samples alongside the standard curve. The assay integrates with standard plate-reader workflows and downstream data analysis used in laboratory research. Researchers handling cell culture supernatant, tissue homogenate, and clarified samples from rat studies can adapt the protocol within the assay's validated range. The kit is supplied for research use only and is suitable for studies investigating extracellular matrix remodeling, renal pathology, and tissue fibrosis.