Rat COL3 alpha1 (Collagen Type III Alpha 1) ELISA Kit (RTES00817)

This Assay Genie kit targets the COL3A1 gene product, catalogued under Uniprot accession P13941, with pre-coated plates that capture native and recombinant rat type III collagen alpha-1 chains. Reconstituted standard vials generate a seven-point curve spanning 10, 5, 2.5, 1.25, 0.625, 0.312, and 0.156 ng/mL for interpolation of unknown specimens. Spike-and-recovery validation in rat-derived matrices yields average recoveries between 84 and 91 percent, while intra-assay CV stays under 10 percent and inter-assay CV under 12 percent. The HRP-streptavidin detection cascade terminates with a TMB substrate incubated for 15-25 minutes at 37 degrees C before a sulphuric acid stop solution is added. Each kit ships with a 12-month shelf life when stored refrigerated at 4 degrees C, with working aliquots of standard remaining stable through repeated freeze-thaw cycles.