Rat COL1A2/Collagen I Alpha 2 ELISA Kit

Assay Genie's Sandwich ELISA Kit is intended for the precise measurement of COL1A2 (Collagen I Alpha 2) levels in rat samples. Compatible sample types include serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, and the kit is intended for storage at 2–8°C for 12 months.

Reported validation data cover linearity at dilutions of 1:2, 1:4, and 1:8 and recovery ranges for serum, EDTA plasma, and heparin plasma. The procedure involves equilibrating reagents, adding standards and samples, sequential incubations with a biotin-labeled detection antibody and HRP-Streptavidin (SABC), TMB-based color development in the dark, and absorbance reading at 450 nm following a stop step.

COL1A2 is a key component of type I collagen that supports the structural integrity of tissues and organs. Dysregulation of COL1A2 expression has been associated with pathological conditions such as fibrosis, arthritis, and skin disorders, supporting the kit's use in research into collagen-related biology, disease progression, and potential therapeutic interventions.