Rat CNTF (Ciliary Neurotrophic Factor) CLIA Kit (RTES00120)

Manufactured by Assay Genie, this chemiluminescence-based ELISA kit is designed for the accurate and reliable detection of Ciliary Neurotrophic Factor (CNTF) in rat samples. The kit is reactive with rat and is validated for use with serum, plasma and other biological fluids. It uses a sandwich-CLIA principle, in which the micro CLIA plate is pre-coated with an antibody specific to Rat CNTF. Standards or samples are added to the wells and combined with the specific antibody, followed by a biotinylated detection antibody and an Avidin-Horseradish Peroxidase (HRP) conjugate. After unbound components are washed away and substrate is added, the relative light unit (RLU) value is measured by a chemiluminescence immunoassay analyzer, with RLU positively associated with the concentration of Rat CNTF.

The kit applies to the in vitro quantitative determination of Rat CNTF concentrations, with a stated assay time of 3.5h, a sensitivity of 7.50 pg/mL and a detection range of 12.50-800 pg/mL. The required sample volume is 100 μL. The manufacturer reports no significant cross-reactivity or interference between Rat CNTF and analogues, and reproducibility is given as both intra-CV and inter-CV being < 15%. CNTF is described as a key neurotrophic factor that plays a critical role in promoting the survival and differentiation of various neuronal populations and is involved in the regulation of neural development, synaptic plasticity and neuroprotection, with dysregulation implicated in various neurodegenerative disorders, supporting its use as a biomarker in developmental biology research.