Rat Clusterin beta PharmaGenie ELISA Kit (SBRS0124)

The Assay Genie competitive immunoassay uses a pre-coated microplate to quantify Clusterin beta (apolipoprotein J) in rat-derived samples. Researchers apply this kit in studies of neurodegeneration, renal injury, and apoptotic cell death, where CLU expression changes serve as biomarkers of tissue stress. Each run includes a biotinylated antigen conjugate and streptavidin-HRP working reagent, and absorbance readings from the chromogenic substrate allow interpolation against the supplied standard curve. Unopened kits should be refrigerated, with longer-term component storage at -20 degrees C to preserve antibody binding activity. Reconstituted standards and biotin conjugate tolerate several freeze-thaw cycles when aliquoted into single-use vials to limit repeated handling. The procedure tolerates both cell culture supernatant and serum matrices, supporting translational work that bridges in vitro cell models with in vivo rat physiology. Investigators in diabetic nephropathy, traumatic brain injury, and reproductive biology have used this reagent to track Clusterin beta dynamics across disease progression.