Rat Cluster Of Differentiation (CD163) ELISA Kit

CD163 functions as a hemoglobin scavenger receptor on the surface of circulating monocytes and mature tissue macrophages, with soluble CD163 levels serving as a research biomarker in studies of inflammation, macrophage polarization, sepsis, hepatic fibrosis, and atherosclerosis in rat disease models. Tracking this marker helps investigators evaluate M2-like macrophage activity across tissue and blood compartments.

This rat CD163 ELISA from Assay Genie is supplied as a 96 assay kit, with reagents sized for full standard curves plus unknown sample measurements. Components include a precoated microplate, biotinylated detection antibody, calibrators, assay buffer, wash concentrate, and substrate. The colorimetric method is read on a standard microplate reader after development, and the workflow follows an in vitro protocol intended for research use only.

Sample matrices accepted by the protocol include serum, EDTA plasma, heparin plasma, and tissue homogenate obtained from rat experimental subjects. Researchers commonly apply it to lysates of spleen, liver, lung, kidney, and adipose tissue, paired with matching peripheral blood draws, to follow CD163 changes across disease progression or after pharmacological intervention.

Unopened kit components should be stored refrigerated and protected from light until first use, with working dilutions handled according to the product insert. Plates and unused reagents are discarded following standard laboratory practice after each run.