Rat Cluster Of Differentiation 19 (CD19) ELISA Kit

The Assay Genie Rat CD19 ELISA Kit enables quantitative in vitro measurement of Cluster of Differentiation 19, a 95 kDa transmembrane glycoprotein of the immunoglobulin superfamily that serves as a defining surface marker of the B-lymphocyte lineage. Because CD19 expression spans the pro-B cell through mature B cell developmental window but is downregulated on terminally differentiated plasma cells, the assay provides a sensitive readout of the mature B-cell compartment in rat lymphoid tissues. Investigators working with autoimmune-prone rat strains, transplant rejection models, and vaccine immunogenicity studies rely on this kit to track B-cell expansion, contraction, and reconstitution in tissue homogenates and cell lysates prepared from spleen, lymph node, bone marrow, and peripheral blood mononuclear preparations. Each kit ships with a pre-coated strip plate, biotinylated detection reagent, streptavidin-HRP working solution, concentrated wash buffer, calibration standard with accompanying diluent, chromogenic substrate, and stop solution. The sealed foil pouch with desiccant safeguards antibody binding activity, and reagents remain stable when refrigerated between uses. Standard protocols call for a small microliter sample volume per well, with results read on a standard laboratory plate reader after substrate incubation. Assay Genie provides a detailed protocol insert specifying recommended dilutions, incubation times, and data analysis guidance tuned for rat-derived matrices, supporting reproducible quantification across experimental cohorts.