Rat Claudin 5 (CLDN5) ELISA Kit

Quantitative measurement of Claudin-5, a transmembrane tight junction protein essential to endothelial barrier integrity, is supported by the Assay Genie Rat Claudin 5 (CLDN5) ELISA Kit using rat tissue homogenates and cell lysates. Claudin-5 is the dominant claudin within the blood-brain barrier, making this kit relevant to investigations of BBB disruption in ischemic stroke models, neuroinflammation studies, and brain microvascular endothelial cell research.

Each kit supports 96 determinations with absorbance-based readout at the conclusion of the immunoreaction sequence. In vitro applications include quantifying CLDN5 expression in cultured rat brain endothelial monolayers under inflammatory challenge, while in vivo work spans cerebral ischemia-reperfusion injury, retinal vasculature assessment, and gastrointestinal mucosal barrier studies where CLDN5 levels are commonly interpreted alongside occludin and ZO-1.

Supplied as a complete kit with pre-coated microplate, detection antibody, standards, and assay buffers, the package insert outlines recommended sample dilution ranges, working solution preparation, and incubation timing. All reagents are intended for research use only and should be stored refrigerated between uses to preserve stability through the stated shelf life.