Rat CKMT1B ELISA Kit

From Assay Genie, this Sandwich ELISA kit is configured for the quantitative detection of CKMT1B in rat samples. Compatible sample types include serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The double-antibody sandwich format is read colorimetrically at 450 nm following TMB substrate development.

Stated performance specifications include a sensitivity of 1.688 ng/mL and a measuring range of 2.813–180 ng/mL. Reported linearity at 1:2, 1:4 and 1:8 dilutions falls within 87–104% for serum, 84–101% for EDTA plasma and 81–98% for heparin plasma. Recovery ranges are 93–103% (serum, average 98%), 86–101% (EDTA plasma, average 92%) and 85–100% (heparin plasma, average 92%).

CKMT1B is described as an enzyme involved in cellular energy metabolism, particularly ATP synthesis in muscle cells, with expression levels that may serve as an indicator of mitochondrial function and energy production in physiological and pathological conditions. The kit is stored at 2–8°C for 12 months, and the assay protocol covers reagent equilibration, a 90-minute primary incubation at 37°C, biotin-labeled detection antibody binding (60 minutes at 37°C), HRP-Streptavidin binding (30 minutes at 37°C), TMB color development in the dark for 10–20 minutes, and absorbance measurement at 450 nm after stop solution is added.