Rat CKLFSF6 (Chemokine Like Factor Superfamily 6) CLIA Kit (RTES00113)

This chemiluminescence-based immunoassay from Assay Genie targets the CKLFSF6 protein (also referenced as CMTM6), a transmembrane chemokine-like factor studied in immune regulation, tumor biology, and cytokine signaling research. The kit is supplied as a 96-well microplate pre-coated with capture antibody, along with standards, detection reagents, and wash buffer to support quantitative measurement of CKLFSF6 in serum, plasma, and other biological fluids.

Standard assay workflow involves adding 50 µl of sample and standard to each well, followed by incubation, washing, and addition of chemiluminescent substrate. Signal is read on a luminometer, with analyte concentration interpolated from a standard curve. Typical intra-assay and inter-assay CV values are reported below 10% and 15%, respectively, supporting reproducible quantitative data.

For best results, store unopened kit components at 2-8°C and avoid freeze-thaw cycles of working reagents. Reconstituted standards should be aliquoted and frozen at -20°C for extended use. This product is intended for research use only and is not approved for diagnostic applications.

Typical research applications include biomarker discovery in immunology, oncology, and inflammation studies, as well as pathway analysis of CMTM6-mediated PD-L1 regulation in cancer cell lines and tissue samples.