Rat Chi3l1 (Chitinase-3-like protein 1) ELISA Kit

From Assay Genie, this Sandwich ELISA kit is designed for the quantitative measurement of rat Chitinase-3-like protein 1 (CHI3L1), a glycoprotein implicated in inflammation, tissue remodeling and cell proliferation. CHI3L1 is also known by synonyms including YKL-40, GP39, CGP-39 and HC-gp39. Elevated CHI3L1 levels have been associated with diseases such as asthma, arthritis and cancer, making it a valuable biomarker for studying disease pathogenesis and potential therapeutic targets. The assay uses a double-antibody sandwich configuration with colorimetric detection and is reactive with rat samples.

Compatible sample types include serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The kit offers a sensitivity of 46.875 pg/mL and a standard curve range of 78.125-5000 pg/mL, with reagents stored at 2-8°C for 12 months.

Reported linearity at 1:2, 1:4 and 1:8 dilutions (n=5) was 91-104%, 83-101% and 88-100% for serum; 85-104%, 85-98% and 81-98% for EDTA plasma; and 87-104%, 86-90% and 89-100% for heparin plasma. Spike-and-recovery across the same matrices ranges from 86-105% (average 93%) in serum, 85-104% (average 93%) in EDTA plasma and 94-104% (average 98%) in heparin plasma. The detected antigen corresponds to UniProt entry Q9WTV1.