Rat Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) ELISA Kit

This quantitative immunoassay kit from Assay Genie enables measurement of rat CX3CL1, a unique chemokine distinguished by its C-X3-C disulfide motif that supports both membrane-anchored adhesion signaling and a soluble chemoattractant form generated through proteolytic cleavage by ADAM-family metalloproteases. The kit is supplied as a complete 96-assay package including pre-coated plate, lyophilized standards, biotinylated detection antibody, streptavidin-HRP conjugate, and substrate reagents.

CX3CL1, also called Fractalkine or neurotactin, is studied across multiple research areas including microglial activation and neuron-glia communication in neuroinflammation models, monocyte and NK cell trafficking in inflammatory disease, fractalkine expression in cardiac tissue following ischemia-reperfusion injury, chronic pain pathways, atherosclerosis progression, and kidney injury models.

The format supports a range of sample matrices including serum-derived, plasma-derived, tissue-derived, and cell-derived preparations such as homogenates, lysates, and cell culture supernatants. Common companion workflows include flow cytometry, immunohistochemistry follow-up, western blot validation, and cell culture supernatant analysis. Refrigerated aliquots should be processed within the storage window specified in the insert.