Rat CDK5 ELISA Kit

Manufactured by Assay Genie, this rat-reactive Sandwich ELISA kit uses a double-antibody format for the quantitative detection of cyclin-dependent kinase 5 (CDK5). The assay covers a detection range of 0.313-20 ng/mL with a sensitivity of 0.188 ng/mL, and is suitable for serum, plasma, cell culture supernatant, cell or tissue lysates, and other liquid samples. Storage is at 2-8°C for up to 12 months.

CDK5 is a key enzyme that regulates neuronal development, synaptic plasticity, and cell migration in the central nervous system, and its dysregulation has been implicated in Alzheimer's disease, Parkinson's disease, and amyotrophic lateral sclerosis, making this kit a useful tool for neuroscience research. The workflow involves equilibrating the TMB substrate and pre-coated plate, loading standards and samples for a 90-minute primary incubation at 37°C, adding a biotin-labeled detection antibody for a 60-minute incubation, adding HRP-Streptavidin for 30 minutes, developing colour with TMB substrate in the dark for 10-20 minutes, and reading absorbance at 450 nm immediately after adding stop solution.