Rat CD28 (Cluster of Differentiation 28) ELISA Kit (RTES01136)

Profiling costimulatory signaling in rat T-lymphocytes is straightforward with this microplate-based ELISA from Assay Genie, which quantitatively detects Cluster of Differentiation 28 (CD28) in research samples. CD28 binds B7-1 (CD80) and B7-2 (CD86) on antigen-presenting cells, transducing the second signal needed for IL-2 production, clonal expansion, and survival alongside T-cell receptor engagement.

Common research applications include tracking T-cell activation in transplantation tolerance models, evaluating CD80/CD86 blockade strategies with CTLA-4-Ig fusion proteins, monitoring vaccine responses, and characterizing immune dysfunction in autoimmune and chronic inflammatory disease models. The kit supports longitudinal sampling in preclinical efficacy and pharmacodynamic studies.

Provided as a 96-assay microplate format, the kit ships with pre-coated wells, biotinylated detection antibody, rat CD28 standards, and substrate solution. Reconstituted standards and detection reagent should be stored refrigerated and protected from light; aliquot standards to minimize freeze-thaw cycles. Intended for research use only and validated for rat-derived sample types.