Rat CCK PharmaGenie ELISA Kit (SBRS0122)

The Assay Genie Rat CCK PharmaGenie ELISA Kit enables quantitative measurement of cholecystokinin (CCK), a 33-amino-acid peptide hormone released by intestinal I-cells in response to nutrient intake. CCK governs satiety signaling, pancreatic exocrine secretion, and gall bladder contraction, making it a key analyte in metabolic, gastrointestinal, and obesity research. Investigators employ this competitive immunoassay to profile CCK concentrations across rat serum and cell culture supernatant specimens in studies of appetite regulation, type 2 diabetes models, bariatric interventions, and gut-brain axis communication. The kit ships with a pre-coated microtitre plate, biotinylated detection reagent, streptavidin-HRP conjugate, lyophilized peptide standards spanning a standard curve, wash buffer concentrate, and stop solution. Each reaction uses a 50 uL sample volume with results interpolated against the included calibration curve. All reagents arrive in individual vials and should be equilibrated to room temperature before running the assay. The microplate format supports high-throughput screening across multiple experimental replicates. Intended for research use only and not validated for clinical diagnostic procedures, the kit pairs well with downstream peptide hormone multiplex panels when profiling enteroendocrine signaling networks.