Rat CCK-8/Cholecystokinin 8 ELISA Kit

Designed for the quantitative measurement of Cholecystokinin-8 (CCK-8) in rat samples, this competitive ELISA from Assay Genie is coated with antigen and uses a colorimetric detection method. The kit is validated for use with serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, offering a sensitivity of 9.375 pg/mL and a working range of 15.625–1000 pg/mL.

The assay relies on a biotin-labelled antibody and HRP-streptavidin detection with TMB substrate, read at 450 nm. Reported linearity across 1:2, 1:4, and 1:8 dilutions spans 89–103% for serum, 87–103% for EDTA plasma, and 86–105% for heparin plasma. Recovery averages 92% in serum, 95% in EDTA plasma, and 91% in heparin plasma. All components should be stored sealed at 2–8°C and not cryopreserved.