Rat Catechol-O-Methyltransferase (COMT) ELISA Kit

Quantitative immunoassay from Assay Genie enabling measurement of Catechol-O-Methyltransferase (COMT) levels in rat-derived biological samples. COMT catalyzes O-methylation of catechol substrates using S-adenosylmethionine as the methyl donor, functioning as a primary inactivation pathway for dopamine, norepinephrine, epinephrine, and dietary catechol compounds. This kit supports neuroscience and pharmacology research, particularly rodent models of Parkinson's disease, schizophrenia, attention deficit investigations, and stress response pathways.

The assay accommodates multiple sample matrices including tissue homogenates from rat brain regions such as striatum, prefrontal cortex, and adrenal medulla, alongside cell lysates and peripheral blood-derived fractions. Each microplate handles up to 96 assays with absorbance-based measurement. Pre-coated capture wells bind rat COMT selectively, and the protocol provides detection reagents and standards requiring reconstitution before use. Applications include pharmacodynamic evaluation of COMT inhibitor compounds in preclinical rodent work, regional methylation capacity assessment across discrete brain nuclei, and behavioral phenotype correlations in genetic models relevant to neuropsychiatric research.